Endogenous Circadian Rhythms in Acetabularia acetabulum.

A complete collection of electronically registered and digitally stored data.

Max-Planck-Institut fuer Zellbiologie 1980 – 1994.

Contents Part 1 (Browser help)

Introduction

Contact Information

DATA presentation

Bibliographical Information

downloads

Last Revised

Introduction

Following the pioneering work of J. Haemmerling, the artificial culture of unicellular giant cells of Dasycladales has uniquely been setup and maintained at the Max-Planck-Institut fuer Zellbiologie by H.G. Schweiger and S. Berger around 1960 in Wilhelmshaven and after 1977 in Ladenburg near Heidelberg.

With the progress of feasible sensor electronics and computing facilities a highly sophisticated laboratory for the study of the circadian rhythm for instance of the oxygen production of Acetabularia was created and operated in Ladenburg (H.G. Schweiger, S .Berger, D. Mergenhagen, H.G. Wallraff, H. Broda, D. Wolff, J. Dirk, Berthel et al. ).

With the adaption of advanced spectral time-series software on a dedicated computer DEC_2020, the accumulated and extended data until 1994 could fully be exploited . (L.von Lindern, S. Berger, D. Mergenhagen, J. Dirk, D. Wolff)

It was Peter Traub’s unfaltering support and the friendly instigation of Franz Halberg, that inspired the intention to present the Ladenburg data in this form.

This data collection owes credit to the effort of so many dedicated scientists and their able assistants, that proper recognition in this place is impossible.

The monograph on Dasycladales by S. Berger and M.J. Kaever will be a splendid companion for the inquiring reader.

In this first part the complete data set is presented in a fashion that allows consistent application of the same evaluation algorithms to all data. No specific association of the data with various experimental conditions and aims of research are intended at this instance.

As an example the data of the analysis of a single cell is detailed and the complete set of ca. 800 data plots is made displayable.

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DATA presentation

The complete set of digitally recorded and stored data for the period 1980 –1994.

1980 – 1990 general research on circadian rhythms in Dasycladales

1990 - 1994 circadian rhythm of oxygen production, influences of temperature, removal of cell nuclei and aging.

A total of about 800 datasets are registered in following pages.

A802901 A810920 A813105 A824140

A831738 A833134 A842007 A850814

A860406 A861013 A870620 A871918

A873014 A874320 A880917 A882508

A890302 A892507 A894411 A900806

A902309 A903311 A910419 A911216

A912113 A912903 A913702 A920302

A920522 A921414 A922716 A924116

A930416 A931313 A932616 A934808

A940408 A941102 A941513 A942713

A943114 A943918 A944718

Each address corresponds to the first of 20 data set’s unique number, which is explained below.

For extended trials take the following test pages:

page10dat, page10int , page10skg which contain the data of A903311.

For corresponding downloads see Browser help

A unique number is assigned to each dataset: AXXYYZZ (xx year, yy week, zz system)

raw data Axxyyzz.dat as recorded in system

pulse data Axxyyzz.pls times of intervention or change of condition

input data Axxyyzz.int equispaced, interpolated raw data

spectral data Axxyyzz.skg spectral (minimum leakage search) data

plot data Axxyyzz.pcx plot of interpolated raw data and spectral data

The algorithm of minimum leakage search is described in L.v.Lindern et al. 1994; where also a detailed discussion of the analysis of the cell A903806 is given. Its plot serves as a frontispiece to this homepage. A first guidance to access these data is given via Browser help.

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A couple of first lines of a dataset set are displayed for the cell A903806.

A903806.dat

FILE B3806 AB 18.09.1990-13:37 - SYSTEM # 6

SAUERSTOFFENTWICKL. - PARALLELE ON-LINE DATENUEBERTRAGUNG

12555 .82958

12556 .818833

12557 .81982

12558 .829793

12559 .831147

12560 .823347

A903806.pls

12600 t1 25

13138 t2

13232 T1 20

13604 T2

13632 T1 27

14180 T2

14208 T1 29

14592 T2

A903806.int

Hlfhr 1.000000 12555.000000 0.000000 F:\CRL\CRLdat\A903806.DAT VAR01

Ncomnt= 3 STOPtim= 14616.0000 hlfhr NDATorg= 1983 MODintpol= 1 NPTot= 2062

comnt: FILE B3806 AB 18.09.1990-13:37 - SYSTEM # 6

comnt: SAUERSTOFFENTWICKL. - PARALLELE ON-LINE DATENUEBERTRAGUNG

comnt: END

0.8295800 0.0000000

0.8188330 0.0000000

0.8198200 0.0000000

0.8297930 0.0000000

0.8311470 0.0000000

0.8233470 0.0000000

0.8116330 0.0000000

0.7879530 0.0000000

0.7580000 0.0000000

0.7795200 0.0000000

A903806.skg

hlfhr 48.000 12555.000 0.000 A903806.INT F:\cr\cr1\A903806.DAT VAR01

hlfhr 1.000 12555.000 0.000 A903806.INT F:\cr\cr1\A903806.DAT VAR01

IDREIF, MOF 0 1 DETR, FILTR 77.000 0.000 IF1MIN, IF1MAX 4 121

NP1ORG: 240 OVERLAPshft: 48 PTS. LEAKG.INCR.: 2 Saturday, 07 February 1998 22:24:04

Period circTIMAX np1 ncount xpow % xpro xres iev*deltlk sttim hlfhr

58.500 30.722 234 1 0.003 60.85 9.165 0.500 12555.000

52.000 37.738 208 2 0.003 59.40 8.147 0.500 12603.000

53.500 47.236 214 3 0.007 76.58 8.382 0.500 12651.000

54.500 26.919 218 4 0.013 81.95 8.538 0.500 12699.000

55.000 18.474 220 5 0.015 80.76 8.617 0.500 12747.000

53.000 9.619 212 6 0.022 84.29 8.303 0.500 12795.000

53.500 48.644 214 7 0.028 87.66 8.382 0.500 12843.000

53.500 48.626 214 8 0.029 90.07 8.382 0.500 12891.000

53.000 11.273 212 9 0.028 90.36 8.303 0.500 12939.000

53.500 49.172 214 10 0.024 85.75 8.382 0.500 12987.000

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unique cell number Axxyyzz :

xx 80 – 94 years, starting on January 1. 00:00 with interval count of 30mins.

yy weeks in year

zz 1 – 20 number of cuvette with electrode (system), > 20 for special setup .

A for closed dataset ( Broda et al. 1983, elementary digitalisation 2 mins)

B indicates piecewise data (same digitalisation as for A)

Back from Axxyyzz

Downloads always to local disk (Ordner oeffnen)

a) Downloading data of single cells can be done using standard browser features.

b) For download of data collections, see contact information.

c) Download of demo-analysis of cell a903806, executable on MS-Win2000 for experienced user. After unzipping read FLOSU2.DOC and start with FLOSU2435.BAT.

d) For the reader of: Lindern L.v., Berger S., Mergenhagen D.. High resolution measurement of circadian Periodicities in Acetabularia . Int. J. Chronobiol. 1994;11:1-20 REGENERATION of TABLE 1 (page 10) is possible. After unzipping read FLOSU1.DOC and start with FLOSU1435.bat.

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Bibliographical Information (only contextually relevant, preliminary quotations )

Mergenhagen D., Schweiger H.-G.. Recording the oxygen production of a single Acetabularia cell for a prolonged period. Expl. Cell. Res. 1973;81:360-4

Broda H., Dirk J., Schweiger H.-G.. On-line data acquisition of long-term measurements of circadian rhythms in individual cells of Acetabularia. Cell Biophys. 1983;5:43-59

Berger S., Dirk J., Lindern L.v., Wolff D., Mergenhagen D.. Temperature dependency of the circadian periodicity in a single cell (Acetabularia). Bot. Acta 1992;105:382-6

Lindern L.v., Berger S., Mergenhagen D.. High resolution measurement of circadian Periodicities in Acetabularia . Int. J. Chronobiol. 1994;11:1-20

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browser help:

Standard browser is Microsoft Internet Explorer 6 and

above with 600x800 or 768x1024 resolution.

Other browsers like Netscape or Opera may need additional adaption.

Make shure to have a backup of your present browser settings.

This page was tested under MS Windows 2000 Professional SR3.

Kodak-Imaging software as part of MS-Win2000 was used for plot display.

Cerious Software was applied for thumbsnail handling and display.

If your browser cannot display following images, try link A903806 nethertheless

and consult sample output lists. (Size of this page plus data is ca. 90 Mb)

Display of a single thumbsnail image

Test an example by mouse klick (left button) to the link A903806 indicated below. (Only the boxed area then is active, left mouse klick on indicated pointer displays plot, corresponding klicks to .int or .skg show data).

Return to last browser-window through browser back-arrow in upper left part of the symbol tray if return arrow is active. If return arrow is not active, close window by klick on X in upper right edge of frame. link A903806 .

A903806.skg

If these frames are ok with you, go to page10int or page10skg .

Look for A903608 for comparison with preceding testpages.

Only separate pages can momentarily been engaged for this purpose.

The content of plots and data files is discussed in DATA presentation.If a more elaborate adjustment of your browser settings seems unavoidable, some of the following notes may be helpful until a system-administrator can be contacted:

a) For the beginning try to sustain annoying pop-up requests of your browser.

b) Make screen adaptions f.i. to 100% or to fullscreen only locally.

c) Engage only single and complete file downloads (see downloads).

d) Check for interference with other graphics applications.

In addition lvlold@aol.com would appreciate notification.

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Pop-up request

oeffnen : display or show data

speichern: store

abbrechen: cancel

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Aknowledgement:

This homepage was generated with the help of PC equipment which was made available from the MPI fuer Zellbiology, Ladenburg. Especially for the conversion and maintanance of the software for spectral analysis, which was developed for a DEC-2060/20, this equipment played and plays a crucial role. The Acetabularia icon on the frontispiece was derived from a photo taken by S. Berger.

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Last Revised:

lvlold, 7.April 2004 13:51 (revision history available from lvlold@aol.com)

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